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Dynamo Flash SYBR Green qPCR Kit

Fast, Sensitive, and Reliable Real-Time PCR Detection

Real-time quantitative PCR (qPCR) is a cornerstone technique for gene expression analysis, diagnostics, and molecular biology research. The Dynamo Flash SYBR Green qPCR Kit provides a fast, accurate, and easy-to-use solution for detecting and quantifying DNA with high sensitivity.

What Is the Dynamo Flash SYBR Green qPCR Kit?

The Dynamo Flash SYBR Green qPCR Kit is a complete master mix designed for real-time PCR applications using SYBR Green dye, which binds double-stranded DNA and fluoresces upon amplification.

Key components include:

  • High-performance DNA polymerase for rapid, accurate amplification

  • SYBR Green dye for sensitive fluorescence detection

  • Optimized buffer and dNTPs for robust performance

  • MgCl₂ and stabilizers for consistent results

The all-in-one format simplifies qPCR setup and ensures reproducible amplification across samples.

Key Features

  1. Rapid qPCR Amplification

    • Optimized for fast cycling protocols, reducing experiment time without compromising sensitivity or specificity.

  2. High Sensitivity and Accuracy

    • Detects even low-abundance targets with precise fluorescence measurements.

  3. Ready-to-Use Master Mix

    • Minimizes pipetting errors and reduces setup time.

  4. Wide Template Compatibility

    • Works with cDNA, genomic DNA, or plasmid DNA.

  5. Robust Performance

    • Produces clean amplification curves and reproducible Ct values for reliable quantification.

Applications

The Dynamo Flash SYBR Green qPCR Kit is ideal for:

  • Gene expression analysis

  • mRNA quantification from cDNA

  • Validation of knockdowns or overexpression studies

  • Diagnostic and clinical research applications

  • High-throughput qPCR assays

How to Use the Dynamo Flash SYBR Green qPCR Kit

  1. Prepare your qPCR reaction

    • Mix template DNA or cDNA with forward and reverse primers and the Dynamo Flash SYBR Green Master Mix.

  2. Program the qPCR instrument

    • Follow recommended cycling parameters for denaturation, annealing, and extension.

  3. Run the qPCR

    • Monitor fluorescence in real time to quantify DNA amplification.

  4. Analyze results

    • Use Ct values and amplification curves to determine target gene expression or copy number.