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Tm calculator for PCR
The calculated Tm for a given primer can vary significantly between different calculation methods. The Tm calculator below utilizes the method Finnzymes has used for optimizing PCR protocols. We recommend this calculator to be used when setting up amplifications with Finnzymes' DyNAzyme, Phire and Phusion DNA Polymerases and DyNAmo qPCR Kits. See the tables below for specific directions for each product.
NOTE: If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site, make sure to calculate the Tm only for the correctly matched sequence.
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Phire® Hot Start II DNA Polymerase Routine and high-throughput PCR faster than ever Read more |
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Phire® Animal Tissue Direct PCR Kit DNA amplification directly from animal tissue Read more |
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DyNAmo™ ColorFlash qPCR Kits Minimize pipetting errors in qPCR reactions! Read more |
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Phusion® Hot Start II DNA Polymerase The number one hot start DNA polymerase is now even betterRead more |
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