Transposon Products Available Separately

Instruments

Consumables

MuA Transposase
F-750	MuA Transposase	0.22 µg/µl, 20 µl
	5x Reaction Buffer	100 µl
F-750C	MuA Transposase concentrated	1.1 µg/µl, 20 µl
	5x Reaction Buffer	100 µl
	MuA Storage Buffer	100 µl

Entranceposons with wild type MuA recognition sites, with a polylinker
F-751	Entranceposon (CamR)
	The marker gene cat confers resistance to chloramphenicol.
	Component of the TGS™ kit (F-700).
F-759	Entranceposon (KanR)
	The marker gene npt confers resistance to kanamycin.
	Component of the TGS™ kit (F-700).
F-764	Entranceposon (TetR)
	The marker gene tet confers resistance to tetracycline.
F-771	Entranceposon (supF)
	The marker gene supF codes for mutated tRNA that recognizes the amber stop codon (UAG) and can suppress a nonsense mutation in an E. coli strain. If the strain carries an amber nonsense mutation in a gene that is required for growth, for example an antibiotic resistance marker in a plasmid, the suppressing tRNA can insert tyrosine at the UAG stop codon, resulting in a full length gene product.

Entranceposons with wild type MuA recognition sites, without the polylinker
F-778	Entranceposon (CamR-3)
	The marker gene cat confers resistance to chloramphenicol.
F-779	Entranceposon (KanR-3)
	The marker gene npt confers resistance to kanamycin.
F-784	Entranceposon (TetR-3)
	The marker gene tet confers resistance to tetracycline.
F-772	Entranceposon (supF-2)
	(See above)
F-774	Entranceposon (lacZ)
	The marker gene lacZ codes for the -polypeptide of ß-galactosidase. Expression of the marker can be utilized for blue-white selection of the insertion clones.
F-775	Entranceposon (lacZ PT)
	Promoter Trap Entranceposon for searching transcribed regions in target DNA. Expression of the promoterless marker gene lacZa requires transcription from a promoter in the target DNA upstream from the insertion site.

*Entranceposons with modified MuA recognition sites containing NotI sites*
F-760	Entranceposon (M1-CamR)
	The marker gene cat confers resistance to chloramphenicol. Component of the MGS™ kit (F-701).
F-762	Entranceposon (M1-KanR)
	The marker gene npt confers resistance to kanamycin. Component of the MGS™ kit (F-701).

Plasmid Entranceposons comprises a series of high-copy number plasmids that can be used for constructing custom Entranceposons. The plasmids contain a copy of the Entranceposon with the polylinker region. In this polylinker area you can easily clone your DNA sequence of interest.

Plasmid Entranceposons
F-765	pEntranceposon (CamR)
	Entranceposon (CamR) (F-751) in pUC19
F-766	pEntranceposon (KanR)
	Entranceposon (KanR) (F-759) in pUC19
F-767	pEntranceposon (TetR)
	Entranceposon (TetR) (F-764) in pUC19
F-773	pEntranceposon (supF)
	Entranceposon (supF) (F-771) in pUC19

Technical Appendix – Transposon Products FAQ – Transposon Products Transposons products references

Entranceposon (CamR), F-751 Entranceposon (CamR-3), F-778 Entranceposon (IacZ), F-774 Entranceposon (IacZ PT), F-775 Entranceposon (KanR), F-759 Entranceposon (KanR-3), F-779 Entranceposon (M1CamR), F-760 Entranceposon (M1KanR), F-762 Entranceposon (supF), F-771 Entranceposon (supF-2), F-772 Entranceposon (TetR), F-764 Entranceposon (TetR-3), F-784 pEntranceposon (TetR), F-767 pEntranceposon (KanR), F-766 pEntranceposon (supF), F-773 pEntranceposon (CamR), F-765

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