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M-MuLV Reverse Transcriptase, RNase H-
High sensitivity enzyme for cDNA synthesis
Note that F-572S and F-572L will be discontinued on March 31, 2012. We recommend an alternative product: RevertAid H Minus Reverse Transcriptase (not available in US). Please contact your local representative for a free sample.
Full list of products to be discontinued.
Description
Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase RNase H- is an RNA-directed DNA polymerase used for synthesizing a complementary DNA strand. Copying is initiated from a primer using either single-stranded RNA or single-stranded DNA template.
M-MuLV reverse transcriptase RNase H- is purified from a strain of E.coli carrying the Reverse Transcriptase gene from M-MuLV, modified by site-directed mutagenesis to eliminate the RNase H activity. M-MuLV RNase H- RT has improved efficiency for full length cDNA synthesis.
Applications
- cDNA synthesis from single-stranded RNA or DNA.
- Multiple transcripts can be analyzed from a single RT-reaction
- Stand alone enzymes are useful when the same cDNA source is used for analysing several different genes.
Advantages
- High sensitivity
- Improved efficiency for full length cDNA synthesis
- Purified from a recombinant source
Components
M-MuLV RT RNase H- is supplied with 10x M-MuLV Buffer.
Reaction buffer concentrate
1x M-MuLV Reverse Transcriptase Buffer: 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 10 mM dithiothreitol.
Storage buffer
50 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 0.1 % Nonidet P40, and 50 % glycerol (v/v).
Storage
Recommended storage temperature -20 °C.
Ordering information
M-MuLV Reverse Transcriptase, |
Size |
Concentration |
|---|---|---|
F-572S |
10 000 U |
200 U/µl |
F-572L |
50 000 U |
200 U/µl |
