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T7 RNA Polymerase
Note that F-617S and F-617L will be discontinued on March 31, 2012. We recommend alternative products: T7 RNA Polymerase or T7 RNA Polymerase, HC. Please contact your local representative for a free sample.
Full list of products to be discontinued.
Description
Finnzymes' T7 RNA Polymerase is a DNA-depended RNA polymerase coded by the bacteriophage T7. This enzyme catalyzes efficient synthesis of single-stranded RNA in 5’→ 3’ direction, and has a stringent specificity for double-stranded T7 promoter sequence.
Applications
- Efficient and selevtive in vitro transcription
- Production of labeled single-stranded RNA molecules for hybridization probes
- Generation of templates for in vitro translation
- Antisense RNA amplification
Contents
T7 RNA Polymerase is supplied with 10x T7 RNA Polymerase Buffer. 1x buffer contains: 40 mM Tris-HCl (pH 7.9 at 25°C), 6 mM MgCl2, 10 mM dithiothreitol and 2 mM spermidine.
Storage buffer
50 mM Tris-HCl (pH 7.9 at 25°C), 1 mM EDTA, 100 mM NaCl, 0.1 % Triton® X-100, 5 mM dithiothreitol and 50 % glycerol.
Storage
Recommended storage temperature -20 °C.
Ordering information
T7 RNA Polymerase |
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F-617S |
3 000 U (50 U / µl) |
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F-617L |
15 000U (50 U / µl) |
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| Reagents | Instruments | Consumables |
