T7 RNA Polymerase

Description

Finnzymes' T7 RNA Polymerase is a DNA-depended RNA polymerase coded by the bacteriophage T7. This enzyme catalyzes efficient synthesis of single-stranded RNA in 5’→ 3’ direction, and has a stringent specificity for double-stranded T7 promoter sequence.

Applications

• Efficient and selevtive in vitro transcription
• Production of labeled single-stranded RNA molecules for hybridization probes
• Generation of templates for in vitro translation
• Antisense RNA amplification

Contents

T7 RNA Polymerase is supplied with 10x T7 RNA Polymerase Buffer. 1x buffer contains: 40 mM Tris-HCl (pH 7.9 at 25°C), 6 mM MgCl₂, 10 mM dithiothreitol and 2 mM spermidine.

Storage buffer

50 mM Tris-HCl (pH 7.9 at 25°C), 1 mM EDTA, 100 mM NaCl, 0.1 % Triton® X-100, 5 mM dithiothreitol and 50 % glycerol.

Storage and stability

Recommended storage temperature -20 °C. Stable for one year from the assay date.

Ordering information