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DyNAmo™ SNP Genotyping Master Mix
Description
Thermo Scientific DyNAmo SNP Genotyping Master Mix delivers fast, high quality SNP genotyping to customers using end-point fluorescence detection on Real-Time PCR instruments. Reliable and reproducible detection of SNP alleles is achieved via a patent pending master mix formulation that offers enhanced discrimination, even when targeting the most challenging amplicons. DyNAmo SNP Genotyping Master Mix has been optimized to offer superior performance when using TaqMan SNP Genotyping Assays or custom designed assays.
Advantages
- Improve your time to results – Patent pending mix formulation delivers fast protocol times (as low as 50 minutes)
- Achieve accurate genotyping results with as little as 0.5 ng DNA
- A unique two color system aids sample set up procedures
- Consistent performance for high throughput laboratories – Pre-pipetted reactions stable for 3 days at 4°C
- Validated for use on industry leading instruments, including Thermo Scientific PikoReal Real-Time PCR System
Applications
- Typing of disease loci
- Mutation analysis
- Association studies
- Epidemiology studies
DyNAmo SNP Genotyping Master Mix delivers superior performance also with challenging amplicons
The performance of DyNAmo SNP Genotyping Master Mix was compared against three other industry leading master mixes specifically developed for genotyping protocols. All Master Mixes tested delivered an acceptable call rate for an assay targeting a LAC2 SNP (rs4988235) but for a more challenging amplicon (NOTCH2 SNP; rs2453042), two of the four mixes failed to successfully call the correct genotypes.
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Accurate genotyping results over a range of template concentrations
Allelic discrimination plot of 12 human genomic DNA samples, generated using a custom designed hydrolysis probe assay targeting a SNP in NOTCH2 (rs2453042), and DyNAmo SNP Genotyping Master Mix. DyNAmo SNP Genotyping Master Mix has been demonstrated to provide accurate genotyping results within a range of 1-10 ng of source DNA, and in this particular data set, accuracy was maintained with an input level of 0.5 ng of gDNA.
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Achieve improved call rates compared to non-optimized master mix for probe chemistry
The performance of DyNAmo SNP Genotyping Master Mix versus a non-optimized master mix for probe chemistry was assessed. Allelic discrimination plots displayed were generated from 21 human genomic DNA samples (5 ng per sample), using custom designed hydrolysis probe assays targeting a SNP in NOTCH2 and TXB18 (rs2453042 and rs215938, respectively). Although comparable call rates were observed when comparing TXB18 data generated from the two different master mixes detailed, when using the non-optimized master mix, analysis of the NOTCH2 SNP resulted in unusable data. Optimized DyNAmo SNP Genotyping Master Mix offers more consistent results when compared to master mixes not optimized specifically for genotyping applications.
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Components
DyNAmo™SNP Genotyping Master Mix |
F-480S | F-480L |
F-480XL |
|---|---|---|---|
2x DyNAmo SNP Genotyping Master Mix (contains a hot start Tbr DNA polymerase, optimized PCR buffer, MgCl2, dNTP mix including dUTP) |
2 × 1.25 ml (200 reactions of 25 μl) |
10 × 1.25 ml (1000 reactions of 25 μl) |
4 × 12.5 ml (4000 reactions of 25 μl) |
50x ROX passive reference dye |
1 x 250 µl | 1 x 1.25 ml |
2 x 1.25 ml |
| 40x Sample Buffer with yellow dye | 1 ml | 2 x 1 ml | 1 x 10 ml |
Shipping and storage
DyNAmo SNP Genotyping Master Mix is shipped on gel ice. Upon arrival, store all the components at -20°C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the product.
Ordering information
DyNAmo™ SNP Genotyping Master Mix |
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|---|---|
F-480S |
200 reactions (25 µl each) |
| F-480L | 1000 reactions (25 µl each) |
F-480XL |
4000 reactions (25 µl each) |
