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DyNAmo™ Probe qPCR Kit
Description
DyNAmo™ Probe qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The master mix contains all reagents needed for qPCR. Only template DNA, PCR primers and probe need to be added by the user. The 2x master mix includes a hot start Thermus brockianus DNA polymerase.
ROX™ passive reference dye is also supplied with the kit in a separate tube for instruments that require ROX normalization.
DyNAmo Probe qPCR Kit is compatible with all block-based and capillary-based real-time qPCR instruments.
Advantages
- Specificity and sensitivity: Combines probe chemistry with optimized reagents.
- Easy to use: 2x master mix and optimized protocols.
- Broad dynamic range: Delivers a dynamic range of up to eight orders of magnitude.
Broad dynamic range and sensitivity
Human papilloma virus genome was cloned into a plasmid. Dilution series from 108 to 10 copies was prepared to be used as a standard DNA. A 76 bp fragment was amplified with F-450L DyNAmo™ Probe qPCR master mix and detected with a HEX-BHQ labeled TaqMan® probe. Reactions were setup on ABI 7500 Real Time PCR System (Applera Corporation, Inc.). The slope of the standard curve was -3.363 and the correlation coefficient R2 was 0.9997.
Components
DyNAmo™ Probe qPCR Kit |
F-450S |
F-450L |
|---|---|---|
2x Master Mix (containing hot start Thermus brockianus DNA polymerase, optimized PCR buffer, MgCl2, dNTP mix including dUTP) |
1 x 1 ml (for 40 reactions of 50 µl volume or 100 reactions of 20 µl volume) |
5 x 1 ml (for 200 reactions of 50 µl volume or 500 reactions of |
50x ROX™ passive reference dye |
1 x 50 µl |
1 x 250 µl |
Storage and stability
DyNAmo Probe qPCR Kit is shipped in gel ice. Upon arrival store all kit components at -20 ºC. The DyNAmo Probe qPCR Kit is stable for six months from the date of packaging when stored and handled properly.
After use, the leftover thawed 2x master mix can be refrozen and stored at -20 °C.
Ordering information
DyNAmo™ Probe qPCR Kit |
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|---|---|
F-450S |
40 reactions (50 µl each) |
F-450L |
200 reactions (50 µl each) |
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| Reagents | Instruments | Consumables |
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Instruction manual for DyNAmo™ Probe qPCR Kit | ||
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Quick guide for ABI real-time PCR instruments | ||
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Brochure for DyNAmo™ qPCR Kits | ||
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Principles of qPCR Basics of chemistries, instruments, assays and main applications Order a free copy! |
