DyNAmo™ Flash Probe qPCR Kit

Description

DyNAmo™ Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.

DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.

When using RNA as starting material, we recommend DyNAmo cDNA Synthesis Kit (F-470) to ensure high-quality results.

Advantages

• Extremely fast protocols: combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2x master mix and optimized protocols
• Validated with all major real-time PCR instruments
• Licenced for qPCR

Time savings with both fast and conventional real-time PCR instruments

13 probe-based qPCR kits from 10 suppliers were tested according to suppliers’ recommendations. DyNAmo Flash Probe qPCR Kit delivers results faster than any other kit tested. Similar time savings are achieved with DyNAmo Flash qPCR Kit for SYBR Green chemistry.

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Efficient amplification across a broad range of template concentrations

Amplification of a 118 bp amplicon from Salmonella typhimurium InvA gene with DyNAmo Flash Probe qPCR Kit (A: combined annealing and extension 15 s, total run time 67 min) and a kit from a major supplier (B: combined annealing and extension 1 min, total run time 106 min). Reaction setup and cycling was performed as recommended by the suppliers. The results show that DyNAmo Flash Probe qPCR Kit delivers higher amplification efficiency with shorter analysis time.

Components

DyNAmo™ Flash Probe qPCR Kit	F-455S	F-455L
2x master mix (contains hot start Tbr DNA polymerase, optimized PCR buffer, MgCl2 and dNTP mix including dUTP)	1 x 1ml (sufficient for 40 reactions 50 µl each or 100 reactions 20 µl each)	5  x 1ml (sufficient for  200 reactions 50 µl each or 500 reactions 20 µl each)
50x ROX passive reference dye	1 x 50 µl	1 x 250 µl

Storage and stability

DyNAmo Flash SYBR Green qPCR Kit is shipped on gel ice. Upon arrival, store all kit components at -20 °C. When using the 2x master mix, the leftover thawed mix can be refrozen and stored at -20 °C without affecting the performance of the kit.

The kit is stable for six months from the date of packaging when stored and handled properly.

Ordering information

DyNAmo™ Flash Probe qPCR Kit
F-455S	40 reactions (50 µl each) or 100 reactions (20 µl each)
F-455L	200 reactions (50 µl each) or 500 reactions (20 µl each)