Phusion^® High-Fidelity PCR Kit

Complete kit with extreme performance for all PCR applications. Part of Thermo Scientific
High Performance PCR!

Description

Thermo Scientific Phusion® High-Fidelity PCR Kit is available in two sizes. The small kit (F-553S) contains all the necessary reagents for performing 50 amplifications in 50 µl volume or 125 amplifications in 20 µl volume. The large kit contains reagents for 200 amplifications in 50 µl volume or 500 amplifications in 20 µl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 20 control reactions in 50 µl volume or 50 control reactions in 20 µl volume.

General information about Thermo Scientific Phusion DNA Polymerases, their advantages and applications can be found on Phusion DNA Polymerase products page.

Phusion^® High-Fidelity PCR Kit components

Component	Concentration	F-553S	F-553L
Phusion DNA Polymerase	2 U/µl	50 U	200 U
5x Phusion® HF Buffer		1 x 1.5 ml	3 x 1.5 ml
5x Phusion® GC Buffer		1 x 1.5 ml	1 x 1.5 ml
dNTP mix	10 mM/each	100 µl	400 µl
MgCl2 solution	50 mM	1.5 ml	1.5 ml
Control lambda template	0.5 ng/µl	40 µl	40 µl
1.3 kb primers	4 µM each	50 µl	50 µl
10 kb primers	4 µM each	50 µl	50 µl
DNA size standard (F-303SD)		200 µl	400 µl
DMSO		0.5 ml	0.5 ml

Storage

Recommended storage temperature -20°C.

Ordering information

Phusion® High-Fidelity PCR Kit	Size
F-553S	50 reactions in 50 µl volume or 125 reactions in 20 µl volume
F-553L	200 reactions in 50 µl volume or 500 reactions in 20 µl volume