Complete PCR protocols in less than half the time

A 600 bp fragment from human genomic DNA was amplified with hot start DNA polymerases from five major suppliers according to each supplier’s recommendations. Due to the unique hot start technology and a special dsDNA-binding domain in the Phire® Hot Start II DNA Polymerase, the PCR protocol was completed up to four times faster than with Taq DNA polymerases utilizing chemically modified or antibody-based hot start technologies (suppliers A-D). The instrument used in the experiment was Piko® Thermal Cycler from Finnzymes.