Amplification of long fragments

1 = 0.6 kb fragment of human glutathione peroxidase 3 gene 2 = 1.0 kb fragment of human glutathione peroxidase 3 gene 3 = 1.5 kb fragment of human cathepsin K gene 4 = 2.7 kb fragment of human beta-2-microglobulin gene 5 = 7.5 kb fragment of humanbeta-globin gene

Five genomic DNA fragments of different lengths were amplified with three different hot start DNA polymerases according to suppliers' recommendations. Phire® Hot Start II DNA Polymerase produced all five amplicons with very high yields. The competing chemically modified and antibody-based hot start DNA polymerases did not amplify the 7.5 kb fragment at all, and the yields for the shorter fragments were significantly lower than with Phire Hot Start II DNA Polymerase. The instrument used in the experiment was Piko® Thermal Cycler from Finnzymes.