DyNAzyme™ EXT PCR Kit

Description

DyNAzyme™ EXT PCR Kit Components

Storage

Ordering information

Complete kit for standard, difficult and long range PCR

Note that F-552S and F-552L will be discontinued on March 31, 2012. We recommend alternative products: Long PCR Enzyme Mix (not available in US) or DyNAzyme EXT DNA Polymerase. Please contact your local representative for a free sample.

Full list of products to be discontinued.

DyNAzyme™ EXT PCR Kit is designed to provide superior performance for long PCR, difficult PCR and for all standard amplifications. The kit is available in two sizes, with complete reagents for 100 or 500 long amplifications. For standard length amplifications, both kits will yield approximately double the number of total reactions. Both kits contain a control template and two primer sets (500 bp and 20 kb) for performing 20 standard or 20 long PCR control reactions.

Component	Concentration	F-552S	F-552L
DyNAzyme™ EXT DNA Polymerase	1 U/µl	200 U	1 000 U (2 x 500 U)
dNTP Mix	10 mM/each	200 µl (2 x 100 µl)	1 000 µl (2 x 500 µl)
Mg²+-free DyNAzyme™ EXT Buffer		1 x 1.5 ml	4 x 1.5 ml
Optimized DyNAzyme™ EXT Buffer		1 x 1.5 ml	4 x 1.5 ml
MgCl₂-solution	50 mM	1 x 1.5 ml	2 x 1.5 ml
Control λ DNA	0.5 ng/ml	40 µl	40 µl
500 bp primers	25 µM/each	20 µl	20 µl
20 kb primers	25 µM/each	20 µl	20 µl
DNA Size Standard
(λ DNA Hind III + ΦX174 DNA Hae III digests)	50 ng/µl of each	400 µl	400 µl
Gel Loading Dye		1 ml	1 ml
DMSO		0.5 ml	0.5 ml

Recommended storage temperature -20°C.

Instruments

Consumables

DyNAzyme™ EXT PCR Kit	Size
F-552S	100 long or 200 standard PCR reactions
F-552L	500 long or 1 000 standard PCR reactions

Tm calculator for PCR Instructions for using DyNAzyme™ DNA Polymerases DyNAzyme™ DNA Polymerase References FAQ – PCR


			Product information for DyNAzyme™ EXT PCR Kit

			Brochure for DyNAzyme™ DNA Polymerases

DyNAzyme™ EXT DNA Polymerase DyNAzyme™ EXT Buffer Pack Detergent-free DyNAzyme™ EXT Buffer