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DyNAzyme™ I DNA Polymerase
Native thermostable DNA polymerase from Thermus brockianus
Note that F-500S and F-500L will be discontinued on March 31, 2012. We recommend alternative products: Taq DNA Polymerase (native, with BSA), Taq DNA Polymerase (native, without BSA) or Taq DNA Polymerase (recombinant). Please contact your local representative for a free sample.
Full list of products to be discontinued.
Description
Native thermostable DyNAzyme™ I DNA Polymerase is isolated and purified from Thermus brockianus, which is a Finnzymes proprietary strain. DyNAzyme I DNA Polymerase has a half-life of 3 hours at 96 °C and it also contains 5’-->3’ exconuclease activity. DyNAzyme I DNA Polymerase is purified free of contaminating endo- and exonucleases.
Advantages
- Better thermal stability than that of Taq DNA polymerase
- Tolerates DMSO better than Taq DNA polymerase
- High purity, no contaminating activities
- Supplied with optimized buffers or with Mg2+-free buffers
- High batch-to-batch consistency
- Easy to use and optimize
- Detergent-free buffer also available
Contents
DyNAzyme™ I DNA Polymerase is supplied with 10x Optimized DyNAzyme™ Buffer. 1x reaction buffer contains: 10 mM Tris-HCl, (pH 8.8 at 25 °C), 1.5 mM MgCl2, 50 mM KCl, 0.1 % Triton® X-100.
Storage buffer
20 mM Tris-HCl (pH 7.4 at 25 °C), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, stabilizers, 200 µg/ml BSA and 50 % glycerol.
Storage
Recommended storage temperature -20 °C.
Ordering information
| DyNAzyme™ I DNA Polymerase with optimized buffer | Size |
Concentration |
|---|---|---|
F-500S |
100 U |
2 U/ µl |
F-500L |
500 U |
2 U/ µl |
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| Reagents | Instruments | Consumables |
