Part of Thermo Fisher Scientific

Webtools for PCR and qPCR

Tm calculator

Analyzes theTm, MW and extinction coefficient of a primer, and gives dilution instructions.

Multiple primer analyzer

Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.

Reaction setup calculators

PCR reaction setup with Thermo Scientific Phusion^® DNA Polymerases

qPCR reaction setup with Thermo Scientific DyNAmo™ qPCR Kits

cDNA synthesis reaction setup with Thermo Scientific DyNAmo™ cDNA Synthesis Kit

qPCR efficiency calculator

Analyzes qPCR amplification efficiency based on the slope of the standard curve.

OD converter

Calculates DNA and RNA concentrations based on measured OD₂₆₀ values.

Copy number calculator

Calculates copy numbers for DNA originating from different organisms.

Fidelity calculator for Phusion^® DNA Polymerases

Compare the error rate of Thermo Scientific Phusion^® High-Fidelity DNA Polymerase with other DNA polymerases


Speed. Fidelity. Yield. Specificity.




	No need for DNA purification




	Reagents		Instruments		Consumables


			Phire® Hot Start II DNA Polymerase Routine and high-throughput PCR faster than ever Read more

			Phire® Animal Tissue Direct PCR Kit DNA amplification directly from animal tissue Read more

			DyNAmo™ ColorFlash qPCR Kits Minimize pipetting errors in qPCR reactions! Read more

			Phusion® Hot Start II DNA Polymerase The number one hot start DNA polymerase is now even betterRead more

© Copyright 2011 Thermo Fisher Scientific Inc.

Ratastie 2, 01620 Vantaa, Finland - Tel. +358 9 329 100 - Fax +358 9 3291 0500

Learn more about other Thermo Scientific Life Science Research Products

Nucleic acid technologies

Protein Research

PCR Reagents and Plastics

High Content Screening

RNAi Technologies

Gene Expression