Part of Thermo Fisher Scientific

Webtools for PCR and qPCR

Tm calculator

Analyzes theTm, MW and extinction coefficient of a primer, and gives dilution instructions.

Multiple primer analyzer

Analyzes different features of multiple primers simultaneously, e.g. Tm, GC content, dimer formation etc.

Reaction setup calculators

PCR reaction setup with Phusion^® DNA Polymerases

qPCR reaction setup with DyNAmo™ qPCR Kits

cDNA synthesis reaction setup with DyNAmo™ cDNA Synthesis Kit

qPCR efficiency calculator

Analyzes qPCR amplification efficiency based on the slope of the standard curve.

OD converter

Calculates DNA and RNA concentrations based on measured OD₂₆₀ values.

Copy number calculator

Calculates copy numbers for DNA originating from different organisms.

Fidelity calculator for Phusion^® DNA Polymerases

Compare the error rate of Phusion^® High-Fidelity DNA Polymerase with other DNA polymerases


Speed. Fidelity. Yield. Specificity.




	No need for DNA purification




	Reagents		Instruments		Consumables


			Phire® Hot Start II DNA Polymerase Routine and high-throughput PCR faster than ever Read more

			Phire® Animal Tissue Direct PCR Kit DNA amplification directly from animal tissue Read more

			DyNAmo™ ColorFlash qPCR Kits Minimize pipetting errors in qPCR reactions! Read more

			Phusion® Hot Start II DNA Polymerase The number one hot start DNA polymerase is now even betterRead more

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