Direct PCR accessories:
Harris Uni-Core™ and Cutting Mat™

Finnzymes’ Direct PCR approach enables PCR directly from source materials without prior DNA purification. Best results are obtained by using a very small amount of sample as starting material. The Harris Uni-Core™ is a convenient tool for cutting very small punch discs from materials such as animal tissue, plant leaves or Whatman FTA® or Whatman 903® cards for Direct PCR. A compatible Harris Cutting Mat™ is used to provide an even cutting surface for the Harris Uni-Core.

How to use the Harris Uni-Core™

Steps for taking a sample with the Harris Uni-Core™

Step 1.	Step 2.	Step 3.	Step 4.

Step 1. While holding the puncher firmly, press the cutting edge downward into the tissue and rotate the puncher between your fingers in opposite directions.

Step 2. Press the plunger to eject the punch disc into a PCR reaction. Make sure that the sample drops into the PCR solution and does not stick into the tube walls.

Step 3. To prevent cross-contamination, it’s important to clean the cutting edge of the puncher between each sample by dipping it into 2 % NaClO solution. Press the plunger up and down a few times.

Step 4. Wipe the cutting edge with a clean paper towel. Remember to rinse the cutting mat as well.

The steps can be repeated for additional samples.

Direct PCR protocols using the Harris Uni-Core™

Instruction manual for Phire® Animal Tissue Direct PCR Kit
Instruction manual for Phire® Plant Direct PCR Kit

Avoiding cross-contamination when using the Harris Uni-Core™

Ordering information

Harris Uni-Core™ and Harris Cutting Mat™ are trademarks of Shunderson Communications Inc.