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Phire® Animal Tissue Direct PCR Kit
Recommended control primer sequences for zebrafish and Drosophila:
zebrafish (378 bp)
Primer # 1 (20-mer) TAT AGC CCA GGG ACA CGA AC
Melting point: 64.17 °C
Primer # 2 (20-mer) CAG CTG CTC CAA AAA CAA CA
Melting point: 64.38 °C
Drosophila (300 bp)
Primer #1 (21-mer) AAG GGC AGT TTT GGT GGA GAT
Melting point: 66.05 °C
Primer #2 (21-mer) AGT GCG ATG CTT GAT GAA AAC
Melting point: 64.44 °C
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